Lorry drivers closed the A40 in to London as they staged a protest against the increasing price of diesel.
Hundreds of vehicles formed a convoy which blocked the road into London, before hauliers delivered a petition to Downing Street.
Drivers in Wales also staged a go-slow protest, causing disruption on the M4 towards Cardiff.
Speaking to the BBC, haulage company boss Peter Carroll, urged the government to give such firms a 25 pence rebate on fuel.
He said: "We are being murdered and you would not walk past a drowning man."
Mr Carroll added that coach firms already receive a rebate and hauliers should be treated in the same way.
Outside Downing Street, fellow haulier Alex Ovenden stated that the purpose of the protest is to "save another UK industry".
Meanwhile, a report by the Confederation of British Industry has found that the current economic slowdown is hitting transport firms hard.
According to the latest Service Sector report, the last quarter saw hauliers experience the fastest fall in business volumes and values for 20 years.
